yakobson part 1

Archiv für die gesamt Virusforschung 1973 Jan; 43(4)
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Effect of host-cell on antiviral action of acetone.

Libshits BA, Yakobson E, Byckovsky AF, Ghendon YZ, Chernos VI

Archiv für die gesamte Virusforschung 1973 Jan; 40(1)
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Study of some stages of poliovirus morphogenesis in MiO cells.

Ghendon Y, Yakobson E, Mikhejeva A

Journal of virology 1972 Aug; 10(2)

Five to seven minutes after addition of (14)C-amino acids to poliovirus-infected MiO cells, radioactivity is found only in 5S virus-specific structures. With increased time of labeling, radioactivity appears in considerable amounts also in the 14S ar... expand abstractea. In the presence of guanidine blocking the transition of 14S particles in subsequent structures in MiO cells, radioactivity is first detected in the 5S zone and then accumulates predominantly in 14S particles. These results indicate that formation of 5S particles and conversion of a part of their protein into 14S particles reflect the earliest stages of the morphogenesis of poliovirus. In contrast to poliovirus-infected HeLa cells, no 73S particles could be detected in MiO cells, nor could 73S structures be detected in poliovirus-infected MiO cells after a short incubation period (20 to 30 min) with radioactive amino acids, although formation of 150S virions was observed. Addition of guanidine to infected MiO cells leads to accumulation of 14S particles; however, 73S particles are not detected. After removal of guanidine, radioactivity increases only in the 150S area. Incubation of 14S particles isolated from the sucrose gradient of the cytoplasmic extract of infected MiO cells resulted in formation of 73S particles. The results obtained show the modification of the late stages of poliovirus morphogenesis in MiO cells. collapse abstract
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Some properties of temperature-sensitive mutants of poliovirus in two cell lines.

Yakobson EA, Mikheyeva AV, Ghendon YZ

Acta virologica 1972 Jul; 16(4)
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Mechanism of antiviral action of acetone on rabbitpox virus replication.

Chernos VI, Libshits BA, Yakobson E, Ghendon YZ

Journal of virology 1972 Feb; 9(2)

Acetone added to the maintenance medium in a 1% concentration reduces the yield of infectious rabbitpox virus in L-cell monolayer cultures by 90 to 97%. This concentration of the inhibitor is not toxic for cells. It was established that there is an i... expand abstractnhibitor-sensitive stage late in the infectious cycle. Acetone exerted no significant influence on production of early viral messenger ribonucleic acid, formation of polyribosomes, deoxyribonucleic acid (DNA) replication, or protein synthesis. In the presence of acetone, the assembly of so-called "acetone particles" occurred. These particles are similar to normal virions by morphological and sedimentation properties but are slightly different from them in buoyant density. The amount of virus-specific DNA and the optical density of the "acetone particles" are the same as those of normal virions despite a 10- to 25-fold difference in the infectivity of the preparations. collapse abstract
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Antigenic specificity of poliovirus-related particles.

Ghendon YZ, Yakobson EA

Journal of virology 1971 Oct; 8(4)

Poliovirus-specific structures with sedimentation constants of 5, 14, 73, and 150S produced in infected HeLa cells possess different antigenic specificities.
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Characterization of some poliovirus temperature-sensitive mutants and poliovirus-related particle formation under nonpermissive conditions.

Mikhejeva A, Yakobson E, Soloviev GY

Journal of virology 1970 Aug; 6(2)

Investigation of 15 poliovirus temperature-sensitive (ts) mutants by using physiological tests [formation of virus-specific antigen and ribonucleic acid (RNA) under nonpermissive conditions] permitted us to divide them into three groups. From each gr... expand abstract